Hélène Loiselle was a Canadian actress living and working in Quebec.

She was born in Montreal and studied acting with Charlotte Boisjoli, François Rozet, Jean Valcourt and Lucie de Vienne during the 1940s. Loiselle joined the theatre troupe Compagnons de Saint-Laurent and performed works by Jean Giraudoux, Racine, Edmond Rostand and Shakespeare. She spent two years in the early 1950s in Paris developing her technique. On her return, she performed in plays that included Chekhov's Three Sisters and Uncle Vanya, Antigone and Victor ou les Enfants au pouvoir.

In 1995, Loiselle received a Prix Gémeaux for her role in the television drama Sous un ciel variable. She also played several witches in the popular Radio Canada television show for children Fanfreluche.

Loiselle married the comedian Lionel Villeneuve; he died in 2000.

In 2006, she received the Prix Denise-Pelletier.

She died at the age of 85 after suffering from Alzheimer's disease for a number of years.