Aina Asif made her acting debut in 2021 with Anjum Shahzad's Pehli Si Muhabbat, where she portrayed the younger version of Maya Ali's character. In 2022, she rose to prominence with her portrayal of a tomboyish sister of Ahad Raza Mir and Junaid Khan's characters in Hum TV's Ramadan special Hum Tum. The same year, she held a role in Asma Nabeel's Pinjra playing a manipulative and cunning school student who is the daughter of Hadiqa Kiani and Omair Rana's characters.

In 2023, her first role was of a girl next door in Abdullah Seja's soap opera Baby Baji, alongside an ensemble cast of Samina Ahmed, Munawar Saeed, Syed Tuba Anwar and Javeria Saud. She is currently playing the leading role of a child bride in Big Bang Entertainment's Mayi Ri.