Aino Seep (February 25, 1925 – July 8, 1982) was an Estonian opera and operetta soprano and stage and film actress.

Aino Seep was born in Tartu in 1925 to parents Daniel Seep and Linda Seep (née Klaos). She was one of two siblings. She attended primary and secondary schools in Tartu before enrolling in the Tartu School of Music (now, the Heino Eller Tartu Music School), studying under tenor and teacher Rudolf Jõks, and graduating in 1949.

In 1949, shortly after graduating from the Tartu School of Music, Seep joined the Vanemuine theatre as an opera and operetta singer, opera soloist, and stage actress. Seep's first role at the Vanemuine was as Adele, Rosalinde's maid in Johann Strauss II's 1874 operetta Die Fledermaus in 1949. This was followed by a 1950 performance as Susanna, the countess's maid in Mozart's 1786 opera buffa The Marriage of Figaro.